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anti frs2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology anti frs2
    Anti Frs2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/17841/FRS2+Antibody/10__1158_slash_1078___0432__ccr___25___4390-131-4-13
    Average 94 stars, based on 158 article reviews
    anti frs2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: A Multikinase Inhibitor AX-0085 Blocks FGFR1 Activation to Overcomes Osimertinib Resistance in Non-Small Cell Lung Cancer.
    Article Snippet: .. Antibodies were used in Western blot analysis as following: anti-hFGFR1 (Cell Signaling, #9740, Danvers, MA, USA), anti-phospho-hFGFR1 (Cell Signaling, #3476), antihFRS2α (Santa Cruz Biotechnology, sc-17841, Dallas, TX, USA), anti-phospho- hFRS2α (Cell Signaling, #3861), anti-hAxl (Cell Signaling, #8661), anti-phospho-hAxl (Cell Signaling, #5724), anti-Akt (Cell Signaling, #9272), anti-phospho-Akt (Cell Signaling, #9271), anti-ERK (Cell Signaling, #9102), anti-phospho-ERK (Cell Signaling, #9101), anti-PCNA (Santa Cruz Biotechnology, sc-56), anti-Ki-67 (Santa Cruz Biotechnology, sc-23900), anti-Caspase-9 (Cell Signaling, #9502), anti-Bcl-2 (Abcam, ab32124, Cambridge, MA, USA), anti-Bax (Santa Cruz Biotechnology, sc-20067), anti-Cyclin E (BD Biosciences, 554182, San Jose, CA, USA), anti-CDK2 (BD Biosciences, 610145), anti-E-cadherin (Cell Signaling, #3195), anti-vimentin (Santa Cruz Biotechnology, sc-6260), anti-N-cadherin (Santa Cruz Biotechnology, sc-393933), and anti-GAPDH (Santa Cruz Biotechnology, sc-32233). .. Osimertinib was purchased from Selleck Chemicals (Houston, TX, USA).

    Article Title: Generation of human induced pluripotent stem cell lines from patients with FGFR2 -linked syndromic craniosynostosis
    Article Snippet: ES: Embryonic Stemcell. .. Applied as Antibody Dilution Supplier IF primary antibodies ES Marker staining Mouse-α-SSEA4 IgG 1:75 Abcam #AB16287 Rabbit-α-NANOG IgG 1:75 Abcam #AB21624 Mouse-α-TRA-1-81 IgM 1:75 Abcam #AB16289 Rabbit-α-OCT4 IgG 1:250 Abcam #AB19857 Mesoderm staining Goat-α-NCAM IgG 1:100 R&D #AF2408 Endoderm staining Goat-α-SOX17 IgG 1:100 R&D #AF1924 Ectoderm staining Mouse-α-β-Tubulin IgG 1:1000 Sigma-Aldrich #T8660 IF secondary antibodies Goat-α-Mouse IgG (H+L) Alexa-546 1:500 Invitrogen #A-11003 Goat-α-Rabbit IgG (H+L) Alexa-488 1:500 Invitrogen #A-11008 Goat-α-Mouse IgM (H+L) Alexa-546 1:500 Invitrogen #A-21045 Donkey-α-Goat IgG (H+L) Alexa-488 1:500 Invitrogen #A-11055 Goat-α-Mouse IgG (H+L) Dylight-594 1:500 Jackson #115-515-166 Western Blotting Primary antibodies Rabbit-α-FGFR2 IgG 1:1000 Cell Signaling #23328 Rabbit-α-Phospho-FGFR (Tyr653/654) 1:1000 Cell Signaling #3471 Rabbit-α-β-Actin IgG 1:1000 Cell Signaling #4970 Rabbit-α-PLCγ1 1:1000 Cell Signaling #2822 Rabbit-α-Phospho-PLCγ1 (Tyr783) 1:1000 Cell Signaling #2821 Mouse-α-FRS2 (A-5) IgG 1:500 Santa Cruz Biotechnologies sc-17841 Rabbit-α-Phospho-FRS2α (Tyr196) 1:1000 Cell Signaling #3864 Seconday antibody Goat-α-Rabbit IgG HRP-linked 1:1000 Cell Signaling #7074 Goat-α-Mouse IgG HRP-linked 1:1000 Cell Signaling #7076 D is ea se M o de ls & M ec ha ni sm s • S up pl em en ta ry in fo rm at io n Table S2. ..

    Article Title: A Multikinase Inhibitor AX-0085 Blocks FGFR1 Activation to Overcomes Osimertinib Resistance in Non-Small Cell Lung Cancer
    Article Snippet: .. Antibodies were used in Western blot analysis as following: anti-hFGFR1 (Cell Signaling, #9740, Danvers, MA, USA), anti-phospho-hFGFR1 (Cell Signaling, #3476), anti-hFRS2α (Santa Cruz Biotechnology, sc-17841, Dallas, TX, USA), anti-phospho- hFRS2α (Cell Signaling, #3861), anti-hAxl (Cell Signaling, #8661), anti-phospho-hAxl (Cell Signaling, #5724), anti-Akt (Cell Signaling, #9272), anti-phospho-Akt (Cell Signaling, #9271), anti-ERK (Cell Signaling, #9102), anti-phospho-ERK (Cell Signaling, #9101), anti-PCNA (Santa Cruz Biotechnology, sc-56), anti-Ki-67 (Santa Cruz Biotechnology, sc-23900), anti-Caspase-9 (Cell Signaling, #9502), anti-Bcl-2 (Abcam, ab32124, Cambridge, MA, USA), anti-Bax (Santa Cruz Biotechnology, sc-20067), anti-Cyclin E (BD Biosciences, 554182, San Jose, CA, USA), anti-CDK2 (BD Biosciences, 610145), anti-E-cadherin (Cell Signaling, #3195), anti-vimentin (Santa Cruz Biotechnology, sc-6260), anti-N-cadherin (Santa Cruz Biotechnology, sc-393933), and anti-GAPDH (Santa Cruz Biotechnology, sc-32233). .. Osimertinib was purchased from Selleck Chemicals (Houston, TX, USA).

    Marker:

    Article Title: Generation of human induced pluripotent stem cell lines from patients with FGFR2 -linked syndromic craniosynostosis
    Article Snippet: ES: Embryonic Stemcell. .. Applied as Antibody Dilution Supplier IF primary antibodies ES Marker staining Mouse-α-SSEA4 IgG 1:75 Abcam #AB16287 Rabbit-α-NANOG IgG 1:75 Abcam #AB21624 Mouse-α-TRA-1-81 IgM 1:75 Abcam #AB16289 Rabbit-α-OCT4 IgG 1:250 Abcam #AB19857 Mesoderm staining Goat-α-NCAM IgG 1:100 R&D #AF2408 Endoderm staining Goat-α-SOX17 IgG 1:100 R&D #AF1924 Ectoderm staining Mouse-α-β-Tubulin IgG 1:1000 Sigma-Aldrich #T8660 IF secondary antibodies Goat-α-Mouse IgG (H+L) Alexa-546 1:500 Invitrogen #A-11003 Goat-α-Rabbit IgG (H+L) Alexa-488 1:500 Invitrogen #A-11008 Goat-α-Mouse IgM (H+L) Alexa-546 1:500 Invitrogen #A-21045 Donkey-α-Goat IgG (H+L) Alexa-488 1:500 Invitrogen #A-11055 Goat-α-Mouse IgG (H+L) Dylight-594 1:500 Jackson #115-515-166 Western Blotting Primary antibodies Rabbit-α-FGFR2 IgG 1:1000 Cell Signaling #23328 Rabbit-α-Phospho-FGFR (Tyr653/654) 1:1000 Cell Signaling #3471 Rabbit-α-β-Actin IgG 1:1000 Cell Signaling #4970 Rabbit-α-PLCγ1 1:1000 Cell Signaling #2822 Rabbit-α-Phospho-PLCγ1 (Tyr783) 1:1000 Cell Signaling #2821 Mouse-α-FRS2 (A-5) IgG 1:500 Santa Cruz Biotechnologies sc-17841 Rabbit-α-Phospho-FRS2α (Tyr196) 1:1000 Cell Signaling #3864 Seconday antibody Goat-α-Rabbit IgG HRP-linked 1:1000 Cell Signaling #7074 Goat-α-Mouse IgG HRP-linked 1:1000 Cell Signaling #7076 D is ea se M o de ls & M ec ha ni sm s • S up pl em en ta ry in fo rm at io n Table S2. ..

    Staining:

    Article Title: Generation of human induced pluripotent stem cell lines from patients with FGFR2 -linked syndromic craniosynostosis
    Article Snippet: ES: Embryonic Stemcell. .. Applied as Antibody Dilution Supplier IF primary antibodies ES Marker staining Mouse-α-SSEA4 IgG 1:75 Abcam #AB16287 Rabbit-α-NANOG IgG 1:75 Abcam #AB21624 Mouse-α-TRA-1-81 IgM 1:75 Abcam #AB16289 Rabbit-α-OCT4 IgG 1:250 Abcam #AB19857 Mesoderm staining Goat-α-NCAM IgG 1:100 R&D #AF2408 Endoderm staining Goat-α-SOX17 IgG 1:100 R&D #AF1924 Ectoderm staining Mouse-α-β-Tubulin IgG 1:1000 Sigma-Aldrich #T8660 IF secondary antibodies Goat-α-Mouse IgG (H+L) Alexa-546 1:500 Invitrogen #A-11003 Goat-α-Rabbit IgG (H+L) Alexa-488 1:500 Invitrogen #A-11008 Goat-α-Mouse IgM (H+L) Alexa-546 1:500 Invitrogen #A-21045 Donkey-α-Goat IgG (H+L) Alexa-488 1:500 Invitrogen #A-11055 Goat-α-Mouse IgG (H+L) Dylight-594 1:500 Jackson #115-515-166 Western Blotting Primary antibodies Rabbit-α-FGFR2 IgG 1:1000 Cell Signaling #23328 Rabbit-α-Phospho-FGFR (Tyr653/654) 1:1000 Cell Signaling #3471 Rabbit-α-β-Actin IgG 1:1000 Cell Signaling #4970 Rabbit-α-PLCγ1 1:1000 Cell Signaling #2822 Rabbit-α-Phospho-PLCγ1 (Tyr783) 1:1000 Cell Signaling #2821 Mouse-α-FRS2 (A-5) IgG 1:500 Santa Cruz Biotechnologies sc-17841 Rabbit-α-Phospho-FRS2α (Tyr196) 1:1000 Cell Signaling #3864 Seconday antibody Goat-α-Rabbit IgG HRP-linked 1:1000 Cell Signaling #7074 Goat-α-Mouse IgG HRP-linked 1:1000 Cell Signaling #7076 D is ea se M o de ls & M ec ha ni sm s • S up pl em en ta ry in fo rm at io n Table S2. ..

    Incubation:

    Article Title: Fibroblast growth factor receptor alterations and resistance mechanisms in the treatment of pediatric solid tumors
    Article Snippet: Proteins separated in gels were transferred to polyvinylidene difluoride (PVDF) membranes (Thermo Fisher) using the iBlot2 Dry Blotting transfer system (Invitrogen) or using the Invitrogen Mini Blot Module and Novex Transfer Buffer (Life Technologies). .. Membranes were blocked in 5% BSA in TBS with 0.1% Tween 20 for one hour at room temperature and then incubated overnight at room temperature with primary antibodies (all from Cell Signaling, Danvers, MA, except as noted below; all antibodies were diluted in TBST blocking buffer to achieve specific concentrations) to total MEK1/2 (9126), phosphorylated MEK1/2 (9154), total ERK1/2 (4695), phosphorylated ERK1/2 (4370), poly-ADP ribose polymerase (PARP) (9542), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (5174S), total FGFR1 (9740;), phosphorylated FGFR1 (2544), total FRS-2 [sc-17841; Santa Cruz Biotechnology (Dallas, TX)], and phosphorylated FRS2 (Try196; 3864S). .. Membranes were then washed 3 times with PBS with 0.1% Tween-20 and incubated for one hour at room temperature with secondary HRP-conjugated anti-rabbit (W401B; 1:5000; Promega, Madison, WI) or anti-mouse (W402B; 1:5000; Promega) IgG antibodies.

    Blocking Assay:

    Article Title: Fibroblast growth factor receptor alterations and resistance mechanisms in the treatment of pediatric solid tumors
    Article Snippet: Proteins separated in gels were transferred to polyvinylidene difluoride (PVDF) membranes (Thermo Fisher) using the iBlot2 Dry Blotting transfer system (Invitrogen) or using the Invitrogen Mini Blot Module and Novex Transfer Buffer (Life Technologies). .. Membranes were blocked in 5% BSA in TBS with 0.1% Tween 20 for one hour at room temperature and then incubated overnight at room temperature with primary antibodies (all from Cell Signaling, Danvers, MA, except as noted below; all antibodies were diluted in TBST blocking buffer to achieve specific concentrations) to total MEK1/2 (9126), phosphorylated MEK1/2 (9154), total ERK1/2 (4695), phosphorylated ERK1/2 (4370), poly-ADP ribose polymerase (PARP) (9542), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (5174S), total FGFR1 (9740;), phosphorylated FGFR1 (2544), total FRS-2 [sc-17841; Santa Cruz Biotechnology (Dallas, TX)], and phosphorylated FRS2 (Try196; 3864S). .. Membranes were then washed 3 times with PBS with 0.1% Tween-20 and incubated for one hour at room temperature with secondary HRP-conjugated anti-rabbit (W401B; 1:5000; Promega, Madison, WI) or anti-mouse (W402B; 1:5000; Promega) IgG antibodies.



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